Scientific Progress. . .
Apr. 19th, 2003 02:29 pm![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png){kind=small}
bronwynrh. . . Goes Boink
Yeah, yeah we all know the refrain by now. Damn cloning. There's *one* way to get this cloning to work that would be easy. Well, easy in that the cloning would only take 3 steps and I'd be chugging away at the business end of the work quickly. It's not easy if the enzyme I'm using is (a) borrowed from another lab, (b) requires some special treatment and (c) may be expired. Today was my third attempt and my conclusion as I look at my results is that we are facing a full combination of (d) all of the above.
So I can wait until Monday, order more enzyme (because you can bet your bootie they don't carry this fancy enzyme in our stockroom) and try again. . . or take the HARD way.
The hard way requires, oh, about 6 or 7 cloning steps, could take a really long time and I won't be chugging away at the business end of the work very quickly at all. Growf. Figures. Juuuuuuust figures.
I want to try the "easy" way just one more time. Maybe somebody else in the building has this enzyme . . . in a batch that was born AFTER 2000.
Plenty of other things to work on. I have several papers and texts to read, a paper review to finish up, a funding application to polish, postdoc positions to peruse and my own paper to write.
And there's always the laundry and the lawn. My lawnmower's not working. I just about ripped out my shoulder yesterday evening when I tried to start it up. The cord came half-way and then decided it wasn't coming the rest of the way with me. Ouch! I guess I'll be borrowing the neighbor's mower (self-propelled, yay!) until I can get mine fixed.
This is me, trying to take charge of my thesis. I don't think I'm being very successful, yet :-/
Yeah, yeah we all know the refrain by now. Damn cloning. There's *one* way to get this cloning to work that would be easy. Well, easy in that the cloning would only take 3 steps and I'd be chugging away at the business end of the work quickly. It's not easy if the enzyme I'm using is (a) borrowed from another lab, (b) requires some special treatment and (c) may be expired. Today was my third attempt and my conclusion as I look at my results is that we are facing a full combination of (d) all of the above.
So I can wait until Monday, order more enzyme (because you can bet your bootie they don't carry this fancy enzyme in our stockroom) and try again. . . or take the HARD way.
The hard way requires, oh, about 6 or 7 cloning steps, could take a really long time and I won't be chugging away at the business end of the work very quickly at all. Growf. Figures. Juuuuuuust figures.
I want to try the "easy" way just one more time. Maybe somebody else in the building has this enzyme . . . in a batch that was born AFTER 2000.
Plenty of other things to work on. I have several papers and texts to read, a paper review to finish up, a funding application to polish, postdoc positions to peruse and my own paper to write.
And there's always the laundry and the lawn. My lawnmower's not working. I just about ripped out my shoulder yesterday evening when I tried to start it up. The cord came half-way and then decided it wasn't coming the rest of the way with me. Ouch! I guess I'll be borrowing the neighbor's mower (self-propelled, yay!) until I can get mine fixed.
This is me, trying to take charge of my thesis. I don't think I'm being very successful, yet :-/
![[identity profile]](https://www.dreamwidth.org/img/silk/identity/openid.png){kind=small}
(no subject)
Date: April 19th, 2003 01:32 pm (UTC)(no subject)
Date: April 19th, 2003 05:49 pm (UTC)I'm starting to wonder if using actual people isn't more complex than using enzymes...
Stupid HSC and its paperwork. (; It's only a survey! What harm could I possibly do? Give them a papercut?